Abstract
Variation in the endospermal protein patterns of seeds amongst and between inland and coastal populations of the dune building grassLeymus arenarius was examined in Iceland. Seeds were collected from six coastal populations and five inland populations in Iceland. Endospermal proteins (prolamins) of seeds were extracted with Tris-buffered 2-propanol (50%) and β-mercaptoethanol (0.5% v/v). We used 8% and 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to separate the prolamin protein bands, which were stained with Coomassie Brilliant Blue R. Coastal populations were differentiated from inland populations on the basis of high frequency of high molar weight (HMW) (110–150 kg mol−1) prolamins. Coastal populations had significantly higher proportions of their seed with more than two HMW prolamins, than those of inland populations. Subtle differences were found among the HMW prolamins of coastal and inland populations indicating limited intrapopulation variation. The dynamic environment of sand dunes probably influences the genetic composition of these populations. The results suggest that seeds of the inland and coastal populations should be treated separately in sand dune reclamation and restoration works
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Ahokas, H. 1992. Endospermal protein diversity, as revealed by electrophoresis and Ig binding on blotted membranes inLeymus arenarius from non-littoral, roadside niches.Ann. Bot. Fenn. 29: 295–304.
Ahokas, H. 2002. Methanol precipitation using universal protein fractionation method for cultivar identification in cereal, pea andBrassica seeds.Seed Sci. Techn. 30: 437–449.
Ahokas, H. & Fredskild, B. 1991. Coexistence and hybridization ofLeymus mollis andL. arenarius in Greenland and demarcation of the species by endospermal prolamins, leymins.Nord. J. Bot. 11: 385–392.
Anamthawat-Jonsson, K., Bragason, B.Th., Bödvarsdóttir, S.K. & Koebner, R.M.D. 1999. Molecular variation inLeymus species and populations.Molecular Ecol. 8: 309–315.
Bjornsson, H. 1992. Jøkulhlaup in Iceland: prediction, characteristics and simulation.Ann. Glaciol. 16: 95–106.
Bond, T.E.T. 1952. Biological flora of the British Isles.Elymus arenarius.J. Ecol. 40: 217–227.
Clarke, S.M. 1964.Some aspects of the autecology of Elymus arenarius L. Ph.D. thesis University of Hull, UK.
Davy, A.J. & Figueroa, M.E. 1993. The colonization of strandlines. In: Miles, J. & Walton, D.W.H. (eds.),Primary succession on land, pp. 113–131. Blackwell Scientific Publications, Oxford, UK.
Du Cros, D.L., Wrigley, C.W. & Blakeney, A.B. 1979. Fractionation of rice-grain proteins by gradient gel electrophoresis and gel isoelectric focusing. Characterization of rice genotypes.Riso 28: 275–284.
Einarsson, M. 1991. Temperature conditions in Iceland 1901–1990.Jökull 41: 1–19.
Freckleton, R.P. & Watkinson, A.R. 2002. Large-scale spatial dynamics of plants: metapopulations, regional ensembles and patchy populations.J. Ecol. 90: 419–434.
Fridriksson, S. 1987. Plant colonization of a volcanic island, Surtsey, Iceland.Arct. Alpine Res. 19: 425–431.
Gao, J.Y., Huang, Y.Y., Deng, J.Y. & Lin, Z.P. 1995. Expression of HRGP gene in the test for tolerance to high salt concentration among wheat cultivars. In: Li, Z.S. (ed.)Proceedings of the Eighth international wheat genetics symposium. pp. 1061–1063. Beijing, CN.
Giles, B.E. & Goudet, J. 1997. A case study of genetic structure in a plant metapopulation. In: Hanski, I. & Gilpin, M.E. (eds.)Metapopulation biology: Ecology, genetics & evolution, pp. 429–454. Academic Press, London, UK.
Gray, A.J. 1987. Genetic change during succession in plants. Colonization succession and stability. In: Gray, A.J., Crawley M.J. & Edwards, P.J. (eds.),The 26th symposium of the British Ecological Society, pp. 273–293. Blackwell Scientific Publications, Oxford, UK.
Gray, A.J. 2002. The evolutionary context: a species perspective. In: Perrow, M.R. & Davy, A.J. (eds.)Handbook of ecological restoration. Vol. 1. Principles of restoration, pp. 66–80. Cambridge University Press, Cambridge, UK.
Greipsson, S. 1991.Population studies of the dune-building grass Leymus arenarius. Ph.D. Thesis, Univ. of East Anglia, Norwich, UK.
Greipsson, S. 2002. Dunes. In: Davy, A.J. & Perrow, M. (eds.)Handbook of ecological restoration. Vol. 2. Restoration in practice, pp. 214–237, Cambridge University Press, Cambridge, UK.
Greipsson, S. & Davy, A.J. 1994a. Germination ofLeymus arenarius and its significance for land reclamation in Iceland.Ann. Bot. 73: 393–401.
Greipsson, S. & Davy, A.J. 1994b.Leymus arenarius. Characteristics and uses of a dune-building grass.Icelandic Agricult. Sci. 8: 41–50.
Greipsson, S. & Davy, A.J. 1995. Seed mass and germination behaviour in populations of the dune-building grassLeymus arenarius.Ann. Bot. 76: 493–501.
Greipsson, S. & Davy, A.J. 1996. Sand accretion and salinity as constraints on the establishment ofLeymus arenarius for land reclamation in Iceland.Ann. Bot. 8: 611–618.
Greipsson, S. & Davy, A.J. 1997. Responses ofLeymus arenarius to nutrients: improvement of seed production and seedling establishment for land reclamation.J. Appl. Ecol. 34: 1165–1176.
Greipsson, S. & El-Mayas, H. 1996. The stabilisation of coastal sands in Iceland. In: Jones, P.S., Healy, M.G. & Williams, A.T. (eds.),Studies in European coastal management, pp. 93–100. Samara Publishing Ltd., Cardigan, UK.
Greipsson, S., Ahokas, H. & Vähämiko, S. 1997. A rapid adaptation to low salinity of inland-colonizing populations of the littoral grassLeymus arenarius.Int. J. Plant Sci. 158: 73–78.
Gudmundsson, M.T., Sigmundsson, F. & Bjornsson, H. 1996. Ice-volcano interaction of the 1996 Gjalp subglacial eruption, Vatnajokull, Iceland.Nature 389: 954–957.
Hurkman, W.J., Fornari, C.S. & Tanaka, C.K. 1989. A comparison of the effect of salt on polypeptides and translatable mRNAs in roots of a salt-tolerant and a salt-sensitive cultivar of barley.Plant Physiol. 90: 1444–1456.
Jefferies, R.L. 1984. The phenotype: its development, physiological constraints and environmental signals. In: Dirzo, R. & Sarukhán, J. (eds.)Perspectives on plant population ecology, pp. 347–358. Sinauer Associates Inc. Publishers, Sunderland, MS, US.
Jerling, L. 1988. Genetic differentiation in fitness related characters inPlantago maritima along a distributional gradient.Oikos 53: 341–350.
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227: 680–685.
Levy, A.A. & Feldman, M. 1988. Ecologeographical distribution of HMW glutenin alleles in populations of the wild tetrapolid wheatTriticum turgidum var.dicoccoides.Theor. Appl. Gen. 75: 651–658.
McCauley, D.E. 1991. Genetic consequences of local population extinction and recolonization.Trends Ecol. Evol. 6: 5–8.
Nevo, E. & Payne, P.I. 1987. Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel.Theor. Appl. Genetics 74: 827–836.
Nevo, E., Beiles, A., Storch, N., Doll, H. & Andersen, B. 1983. Microgeographic edaphic differentiation in hordein polymorphisms of Wild Barley.Theor. Appl. Gen. 64: 123–132.
Nevo, E., Pagnotta, M.A., Beiles, A. & Porceddu, E. 1995. Wheat storage proteins: glutenin DNA diversity in wild emmer wheat,Triticum dicoccoides, in Israel and Turkey. 3. Environmental correlates and allozymic associations.Theor. Appl. Genetics 91: 415–420.
Pagnotta, M.A., Nevo, E., Beiles, A. & Porceddu, E. 1995. Wheat storage proteins: glutenin diversity in wild emmer,Triticum dicoccoides, in Israel and Turkey. 2. DNA diversity detected by PCR.Theor. Appl. Genetics 91: 409–414.
Scheiner, S.M. & Teeri, J.A. 1986. Microhabitat selection and the successional gradient of a forest grass.Can. J. Bot. 64: 734–738.
Scheiner, S.M. & Teeri, J.A. 1987. Rapid genotypic change in a population of the grassDanthonia spicata following disturbance.Can. J. Bot. 65: 1819–1823.
Shapiro, A.L., Vinuela, E. & Maizel, J.V., Jr. 1967. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.Biochem. Biophys. Res. Comm. 28: 815–820.
Stefansson, U. 1961.Hafid (The Ocean). Almenna bokafelagid, Reykjavik, Iceland.
Suominen, J. 1970. OnElymus arenarius (Gramineae) and its spread in Finnish inland areas.Ann. Bot. Fenn 7: 143–156.
Syros, T., Yupsanis, T. & Economou, A. 2003. Fractionation and electrophoretic patterns of storage proteins ofEbenus cretica. A prelimnary survey as a tool in taxonomy.Biol. Plant. 46: 435–443.
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Greipsson, S., El-Mayas, H. & Ahokas, H. Variation in populations of the coastal dune building grassLeymus arenarius in Iceland revealed by endospermal prolamins. J Coast Conserv 10, 101–108 (2004). https://doi.org/10.1652/1400-0350(2004)010[0101:VIPOTC]2.0.CO;2
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DOI: https://doi.org/10.1652/1400-0350(2004)010[0101:VIPOTC]2.0.CO;2